Pik3ca Knockout cell line(E0771)

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location： Home > Products > KO Cell Lines > Pik3ca Knockout cell line(E0771)

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Pik3ca Knockout cell line(E0771) Order

Pik3ca Knockout cell line(E0771)
Catalog#
YKO-M091
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Pik3ca Knockout cell line(E0771)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Pik3ca Knockout cell line (E0771)
Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:4
Species
Mouse
Gene Name
Pik3ca
Gene ID
18706
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 9 (based on transcript Pik3ca-201)
Validation: PCR+Sanger Sequencing
Pik3ca Knockout cell line(E0771) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:E0771		 STR Info (Cell bank)
Cell Line:E0771
Allele1Allele2Allele3Allele1Allele2Allele3
4-2 241.51
【21.3】		245.52
【22.3】		253.62
【24.3】		241.53
【21.3】		245.65
【22.3】		253.71
【24.3】
5-5 347.70
【17】		363.79
【23】		367.79
【24】		347.74
【17】		363.98
【23】
6-4 303.75
【19】		307.67
【20】		311.37
【21】		299.75
【18】		303.76
【19】		311.72
【21】
6-7 346.33
【15】		346.24
【15】
9-2 229.11
【17】		237.22
【19】		229.23
【17】		237.3
【19】
12-1 229.54
【17】		233.65
【18】		229.65
【17】
15-3 204.62
【23.3】		208.74
【24.3】		211.43
【25.3】		204.74
【23.3】		208.87
【24.3】		212.89
【25.3】
18-3 147.69
【15】		151.76
【16】		151.77
【16】		155.79
【17】		159.91
【18】
X-1 404.16
【26】		416.23
【29】		404.14
【26】		416.23
【29】
D4S2408
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Pik3ca gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the E0771 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Pik3ca gene knockout Pik3ca Knockout cell line (E0771) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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